arivis Vision4D Release 3.0

With the biggest Release of arivis Vision4D so far, we bring several new tools enabling users to perform image analysis more efficiently. We have been constantly listening to our customer and implemented their specific requirements in several new features to equip you with a strong tool for your daily work.
A clean and state-of-the-art user interface shines with straightforward user guidance and makes work simple and fast. Based on the arivis Image Core Technology our new arivis Vision4D 3.0 is the ideal software to work with large datasets and get reliable measuring results by means of the unique arivis Analysis Pipeline.

 New Functionalities of arivis Vision4D 3.0


  • New and improved easy-to-use advanced color settings and LUT editor which allows saving & sharing of sophisticated look-up tables with variable spline curves for gamma settings » View Tutorial
  • Improved User Interface
    • Optimized toolbars, icons and layouts of panels
    • Better Zooming on HR-Screens
    • Renaming of many keywords for easier handling of the software, such as time point (former frame), image set (former scope), panel (former palette), objects (former annotations)
    • Improved layout for scaled UI (e.g. 4K screens)
  • New Line Measurements with Intensity Profiles:  
    • Polyline length and intensity measurements in 2D or 3D views
    • Intensity amplitude measurement
    • Intensity peak to peak distance measurement
    • Export of line profile as csv/xls
  • New Object Drawing Tools allowing to paint and erase segments with automatic interpolation between planes » View Tutorial
    • Fast drawing mode with various brush sizes and nudging
    • after object creation additional options for copying, editing and moving (X, Y, Z, Time and across image sets)
  • New and improved manual object creation using Magic Wand in 2D and 3D mode » View Tutorial
    • supports objects with holes (automatic for Magic Wand or Manual Drawing using the Object Draw Tools)
    • With optional automatic hole filling
    • Works in 2D/4D Viewer and in combined split views
    • Option to sculpt and change objects plane wise after object creation
  • Copy, paste and move objects (translate x,y,z), between images sets , or over time
  • Option to change transparency of objects

Image Analysis

  • Membrane based cell detection included in standard analysis package based on ACME /RACE for membrane staining without the need for a nuclear stain. This method may also be used for and also the automatic detection of for label-free samples imaged with Brightfield Microscopy. » View Tutorial
  • Flexible automatic or manual object grouping with single and group statistics and interactive visualization of groups or single objects in the 4D Viewer. » View Tutorial
    This allows the analysis to fit the experimental task. The user can freely specify relationships between different components to obtain more meaningful results. Objects as well as tracks can be grouped according to feature ranges or custom selection. » View Tutorial
  • User-specified custom measurements now allowing algebraic combination of features and counts of objects and groups, eg. for ratio or FRET measurements in 3D.
  • Easy graphical definition of custom Color Setting for object features. By selection of an area directly on a feature xy plot, a specific feature ranges can be visualized in the 4D Viewer similar to gating. It is also possible to use any custom calculated measurements for selection and visualization.
  • Dilate, erode, close and open objects after segmentation
  • Setting of custom surface transparency of segment surfaces
  • Improved Fly Through mode to dive into the 3D data and fly in any direction with optimized speed adjustment.
  • Sample Analysis Pipelines for typical use cases such as cell or particle detection, tracking or colocalization and membrane-based cell detection.
  • Python scripting improvements:
    • Streamlined scripting package structure
    • Complete support for all-new object backend (object types, creating and modifying objects, import/export)
    • Support for native Python packages as NumPy or SciPy
    • Integration of existing Python environments instead of using the delivered environment
    • Complete support of analysis pipeline operations
    • more and improved sample scripts
  • Complete rework of object handling for faster processing and  visualization of a large number of objects and in large data sets
    • Speed improvements of analysis operators (e.g. Blob Finder) with further improved parallelization
    • Improved tagging and filtering options
    • Option to include holes for segmentation
    • Tracking of objects using intensity-based center of mass
    • Option to choose surface calculations to be performed within the pipeline or upon display

…and much more improvements for better ease of use.


  • Heavily improved CZI import
  • Improved LIF support
  • Some fixes for BigDataViewer import

Use Cases

    New standalone module: arivis SIS Converter Pro for 3.0


    With watchdog functionality! Start your overnight image acquisition, and let arivis prepare your data for immediate visualization as soon as your experiment has completed.

    arivis SIS Converter Pro features:

    • Plane by plane, channel by channel raw file conversion directly to SIS format
    • Unattended automatic file conversion and import
    • Automatically add newly generated images to existing SIS file
    • Faster and automatic file conversion process from image acquisition to SIS format
    • Multiple configurable import sources for file conversion